Composite

Part:BBa_K4613302:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


pET-29a(+)-T3-M-CPA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1062
    Illegal BamHI site found at 1007
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75


Design Notes

We attached 6*his tag to the N-terminus of T3-M-CPA to get purified T3-M-CPA.


Source

NAU-CHINA

References